Computational Comparative Genomics of Eu- and Prokaryotes

With the rapid advent of worldwide genome projects, scientists are confronted with a tremendous increase in the amount of novel information. However, the gap between data collection and interpretation is also growing. For this reason, genome databases contain a wealth of information which is accessible, but which may remain obscured. Much can be learned from comparing different genomes, as genomes of distant organisms may still encode proteins with high sequence similarity. The order of genes (colinearity) in genomes may also be conserved to some extent. We have employed both these observations to create a multi-functional, computational analysis system (genomeSCOUT), which allows for rapid identification and functional characterization of genes and proteins through genome comparison. Using a number of independent methods, information about different levels of protein homology (concerning e.g. orthologs and clusters of orthologous groups, COGs), as well as protein families and gene order, is collected and stored in several databases. These databases are then used for interactive comparison of genomes and subsequent analysis. genomeSCOUT was extensively used for the analysis of two Listeria genomes (Figure 1; [1]). A free demo version of the genomeSCOUT / Listeria server is accessible at: http://GS-Listeria.lionbioscience.com/. genomeSCOUT can easily be extended to allow the analysis of any number of single or multiple,